WebMar 13, 2024 · Steroidogenesis – Human cell line (H295R) Aromatase – Human recombinant microsomes In vivo Uterotrophic (rat) Hershberger (rat) Pubertal female (rat) Pubertal male (rat) Amphibian metamorphosis (frog) Fish short-term reproduction Basis for Assay Selection for the Tier 1 Screening Battery WebThe human H295R adreno-carcinoma cell line, used for the assay, expresses genes that encode for most of the major enzymes and functional proteins in the steroidogenic pathway (e.g. StAR; CYP11A1/scc; CYP11/18; CYP17; CYP19; CYP21; 3-hydroxysteroid dehydrogenase) (Harvey and Everett, 2004).
Multi-Laboratory Validation of the H295R Steroidogenesis …
Webas a screen for chemical effects on steroidogenesis, specifically the production of 17β-estradiol (E2) and testosterone (T) was carried out in a multi–step process. The H295 assay has been optimized and validated (2)(3)(4). 2. The objective of the H295R Steroidogenesis Assay is to detect substances that affect WebEPA and OECD test guidelines for the H295R steroidogenesis assay to detect potential perturbation of estradiol (E2) and testosterone (T) synthesis are designed for low … did pink do the super bowl
Si Wang - Principal Scientist - Scientific Affairs
WebHere, we report on the validation of an in vitro assay, the H295R steroidogenesis assay, to detect chemicals with the potential to inhibit or induce the production of the sex steroid … Websteroidogenesis, specifically the production of 17β-estradiol (E2) and testosterone (T) was carried out in a multi–step process. The H295R assay has been optimized and validated (2)(3)(4)(5). 2. The objective of the H295R Steroidogenesis Assay is to detect substances that affect production of E2 and T. WebAug 24, 2024 · H295R is a human adreno-carcinoma cell line that expresses the genes encoding these key steroidogenesis enzymes. This means the H295R cell system … did pink and her husband get a divorce